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Collection Publication Age- and tumor subtype-specific breast cancer risk estimates for CHEK2*1100delC carriers(American Society of Clinical Oncology, 2016) Schmidt, Marjanka K; Hogervorst, Frans; van, Hien Richard; Cornelissen, Sten; Broeks, Annegien; Adank, Muriel; Meijers, Hanne; Waisfisz, Quinten; Hollestelle, Antoinette; Schutte, Mieke; van, den Ouweland Ans; Hooning, Maartje; Andrulis, Irene L; Anton-Culver, Hoda; Antonenkova, Natalia N; Antoniou, Antonis; Arndt, Volker; Bermisheva, Marina; Bogdanova, Natalia V; Bolla, Manjeet K; Brauch, Hiltrud; Brenner, Hermann; Brüning, Thomas; Burwinkel, Barbara; Chang-Claude, Jenny; Chenevix-Trench, Georgia; Couch, Fergus J; Cox, Angela; Cross, Simon S; Czene, Kamila; Dunning, Alison; Fasching, Peter A; Figueroa, Jonine; Fletcher, Olivia; Flyger, Henrik; Galle, Eva; García-Closas, Montserrat; Giles, Graham G; Haeberle, Lothar; Hall, Per; Hillemanns, Peter; Hopper, John L; Jakubowska, Anna; John, Esther M; Jones, Michael; Khusnutdinova, Elza; Knight, Julia A; Kosma, Veli-Matti; Kristensen, Vessela; Lee, Andrew; Lindblom, Annika; Lubinski, Jan; Mannermaa, Arto; Margolin, Sara; Meindl, Alfons; Milne, Roger L; Muranen, Taru A; NBCS, Investigators; Newcomb, Polly A; Offitt, Kenneth; Park-Simon, Tjoung-Won; Peto, Julian; Pharoah, Paul; Robson, Mark; Rudolph, Anja; Sawyer, Elinor J; Schmutzler, Rita K; Seynaeve, Caroline; Soens, Julie; Southey, Melissa C; Spurdle, Amanda; Surowy, Harald; Swerdlow, Anthony; Tollenaar, Rob AEM; Tomlinson, Ian; Trentham-Dietz, Amy; Vachon, Celine; Wang, Qin; Whittemore, Alice S; Ziogas, Argyrios; van, der Kolk Lizet; Nevanlinna, Heli; Dörk, Thilo; Bojesen, Stig; Easton, Douglas; Law Enforcement; Law and Development; Personal Rights & Property Rights; Government & Law; Business & LawItem DSpace 7 Lightning Tutorial: GDPR and Data Privacy(2020-10-08) Luyten, BramIntroduction to DSpace 7 data privacy features: - Cookie consent - End User Agreement - Deleting EPeople to comply with the right to be forgottenPublication Editorial, Volume 7, Issue 2(2016) Archuleta, Kristy L.This issue features four articles, two profiles, and one book review. Each article adds a new contribution to the field of financial therapy. First, Dr. Asebedo applies a conflict resolution framework to money arguments. Next, Drs. Rea, Zuiker, and Mendenhall explore financial management practices among emerging adult couples. In the third paper, Drs. Ann Woodyard and Cliff Robb help to add further description of financial satisfaction. Then, Dr. Russell James offers a unique theoretical analysis of mortality salience and financial decisions. This issue also features a practitioner profile of Beth Crittenden and a scholar profile of Sarah Asebedo. Finally, we conclude with a review by Neal VanZutphen about a book entitled, The Seven Principles for Making Marriage Work.Publication In vitro and in vivo stability of recombinant plasmids in a vaccine strain of Salmonella enterica var. Typhimurium(2003-07-15) Simmons, CameronThis study examined the ability of different plasmid vectors encoding H(C) fragment, the non-toxic binding portion of tetanus toxin, to be stably retained by Salmonella enterica var. Typhimurium (Salmonella typhimurium) vaccine strain BRD509 and, upon immunisation, to induce an antibody response against the carried antigen. The H(C) fragment expression cassette containing the transcription/translation signals, H(C) fragment open reading frame and the downstream TrpA terminator, was excised from pTETtac4 and incorporated into the plasmids pIC20H, pBR322, pACYC184 and pRSF1010. The resulting constructs were transferred into attenuated S. typhimurium, BRD509, and the level of H(C) fragment expression was examined by Western blot analysis. The relative stability of each plasmid in S. typhimurium was determined in vitro in the absence of antibiotic selection, and in vivo following immunisation. The ability of each H(C) fragment-expressing strain to induce lipopolysaccharide- and tetanus toxoid-specific antibody responses was assayed by an enzyme-linked immunosorbent assay. These studies showed that all the vaccine vector constructs, except the S. typhimurium carrying the expression vector based on pIC20H, were able to elicit a high titre immune response. The level of tetanus toxoid-specific antibody induced by S. typhimurium directly correlated with the level of in vitro and in vivo stability of the H(C) fragment expression plasmid carried by the bacterium, and not with an increased copy number of the parent plasmid vector.Publication An In-Depth Analysis of Original Antigenic Sin in Dengue Virus Infection(2011-01-01) Simmons, CameronThe evolution of dengue viruses has resulted in four antigenically similar yet distinct serotypes. Infection with one serotype likely elicits lifelong immunity to that serotype, but generally not against the other three. Secondary or sequential infections are common, as multiple viral serotypes frequently cocirculate. Dengue infection, although frequently mild, can lead to dengue hemorrhagic fever (DHF) which can be life threatening. DHF is more common in secondary dengue infections, implying a role for the adaptive immune response in the disease. There is currently much effort toward the design and implementation of a dengue vaccine but these efforts are made more difficult by the challenge of inducing durable neutralizing immunity to all four viruses. Domain 3 of the dengue virus envelope protein (ED3) has been suggested as one such candidate because it contains neutralizing epitopes and it was originally thought that relatively few cross-reactive antibodies are directed to this domain. In this study, we performed a detailed analysis of the anti-ED3 response in a cohort of patients suffering either primary or secondary dengue infections. The results show dramatic evidence of original antigenic sin in secondary infections both in terms of binding and enhancement activity. This has important implications for dengue vaccine design because heterologous boosting is likely to maintain the immunological footprint of the first vaccination. On the basis of these findings, we propose a simple in vitro enzyme-linked immunosorbent assay (ELISA) to diagnose the original dengue infection in secondary dengue cases.Publication Site-directed mutagenesis of intimin alpha modulates intimin-mediated tissue tropism and host specificity(2001-04-01) Simmons, CameronThe hallmark of enteropathogenic (EPEC) and enterohaemorrhagic (EHEC) Escherchia coli adhesion to host cells is intimate attachment leading to the formation of distinctive 'attaching and effacing' lesions. This event is mediated, in part, by binding of the bacterial adhesion molecule intimin to a second bacterial protein, Tir, delivered by a type III secretion system into the host cell plasma membrane. The receptor-binding activity of intimin is localized to the C-terminal 280 amino acids (Int280) and at least five distinct intimin types (alpha, beta, gamma, delta and epsilon) have been identified thus far. In addition to binding to Tir, intimin can also bind to a component encoded by the host. The consequence of latter intimin-binding activity may determine tissue tropism and host specificity. In this study we selected three amino acids in intimin, which are implicated in Tir binding, for site-directed mutagenesis. We used the yeast two-hybrid system and gel overlays to study intimin-Tir protein interaction. In addition, the biological consequences of the mutagenesis was tested using a number of infection models (cultured epithelial cells, human intestinal explants and a mouse model). We report that while an I237/897A substitution (positions numbered according to Int280alpha/whole intimin alpha) in intimin alpha did not have any affect on its biological activity, a T255/914A substitution attenuated intimin activity in vivo. In contrast, the mutation V252/911A affected tissue targeting in the human intestinal explant model and attenuated the biological activity of intimin in the mouse model. This study provides the first clues of the molecular basis of how intimin mediates tissue tropism and host specificity.Publication From Visual Semantic Parameterization to Graphic Visualization(IEEE, 2013-06-28) Zeng, XinVisualizing natural language description is a difficult and complex task. When dealing with the process of generating images from natural language descriptions, we firstly should consider the real world and find out what key visual information can be extracted from the sentences which represents the most fundament concepts in both virtual and real environments. In this paper, we present the result of a prototype system called 3DSV (3D Story Visualiser) that generates a virtual scene by using simplified story-based descriptions. In particular, we describe the methodology used to parameterize the visual and describable words into XML formatted data structure. Then we discuss how to interpret the parameterized data and create an interactive real-time 3D virtual environment.Publication Our Economy: Towards a new prosperity(Oxfam GB, 2013-06-20) Trebeck, Katherine; Francis, Stuart; Test, Test; Parthasarathy, PrabhaFor too many Scots, the existing economic model is failing. Far from improving their lives, it traps them in a cycle of economic hardship. Yet it is possible to overcome poverty, both in Scotland and across the UK - many of the solutions already exist, hidden within the very communities hit hardest by an economic model that worships at the altar of ‘economic growth’. The extraordinary work of our partners in Scotland has helped frame this report, where we hope to show how allocating resources in a more effective and sustainable way can deliver lasting change. In this paper we argue that the Scottish economy must pursue policies which deliver for the people, and policy-makers must play a central and driving role as underwriters of community solutions. Some of our recommendations include: - Build on the National Performance Framework and the Oxfam Humankind Index to create a better way of measuring our collective prosperity. - Create a Poverty Commissioner to ensure spending decisions are poverty proofed and to support communities to challenge Government policies and private sector actions that do not contribute to socio-economic equality. - Employers in the public and private sectors should pay a living wage. - Tax havens, offshore earnings and loopholes which allow avoidance, should be pursued and closed. Business support, corporate social responsibility awards and government plaudits should be contingent on companies meeting their tax obligations. - A Sustainable Livelihoods Approach (SLA) to social protection should be pursued. This would recognise complex barriers to work, gender differences and caring responsibilities as well as rewarding individuals’ range of skills and contributions (including activities that deliver social benefit but are currently insufficiently valued by the market). - Funding is required to make it easier for deprived communities to own assets for local benefit. As part of a socio-economic duty, council staff should support deprived communities prepare for ownership, with upfront grants enabling communities to assess the merits of an opportunity.